Multienzyme Cascade Bioreactor for a 10 min Digestion of Genomic DNA into Single Nucleosides and Quantitative Detection of Structural DNA Modifications in Cellular Genomic DNA.

Identification and quantification of chemical modification of DNA provides important information about changes in genomic DNA, for example, epigenetic modifications and abnormal DNA lesions. In this layer, which is needed to digest the genomic DNA strands into a single nucleoside, facilitating mass spectrometry analysis. However, the rapid digestion of DNA as supramacromolecule of several million Dalton (molecular weight) into a single nucleoside remains very challenging.

Here, we constructed a bioreactor Benzonase move capillaries and subsequently immobilized tandem coupled with snake venom phosphodiesterase and alkaline phosphatase capillary bioreactor to form a novel three-enzyme cascade bioreactor (BenzoSAC bioreactor). In this construction, the enzyme selected moving to synthetic porous monolithic silica capillary.

With tailor-made porous structure and high immobilized capacity and high-level Benzonase digestion, genomic DNA of> 99.5% can be digested into a single nucleoside within just 10 minutes when passing through the bioreactor BenzoSAC by the injection pump. In contrast, traditional digestion requires 8-24 hours. With offline clutch benzoSAC bioreactor with chromatography-tandem mass spectrometry liquid, we detected a 5-hydroxymethylcytosine, the main oxidation product of epigenetically importance of 5-methylcytosine in genomic DNA isolated from cancer stairs (T24) cells. The BenzoSAC bioreactor newly synthesized and proposed mass spectrometry detection promising for the rapid identification and analysis of structural modifications in DNA.


Viruses that infect thermophilic Archaea that is unique in both structure and genetics. Lemon-shaped fuselloviruses that infects members of the order Sulfolobales, grows optimally at 80 ° C and pH 3-are some viruses that most ubiquitous and best studied of Archaea thermoacidophilic. Nonetheless, there is still much to be learned about this virus. In order to investigate the evolution of fusellovirus, we have isolated and characterized a new fusellovirus, Sulfolobus spindle-shaped virus 10 (previously SSV-L1). Comparative genomic analysis highlights the significant similarities with both SSV8 and SSV9, as well as the conservation of promoter elements in Fuselloviridae.

Multienzyme Cascade Bioreactor for a <em>10</em> min Digestion of <em>Genomic</em> DNA into Single Nucleosides and Quantitative Detection of Structural DNA Modifications in Cellular <em>Genomic</em> DNA.
Multienzyme Cascade Bioreactor for a 10 min Digestion of Genomic DNA into Single Nucleosides and Quantitative Detection of Structural DNA Modifications in Cellular Genomic DNA.

Comparison of genomic and transcriptome analysis of Lactobacillus rhamnosus ATCC 11443 and mutant strains SCT- 10 – 10 -60 to increase the production capacity of L-lactic acid.

The mechanism for the production of L-lactic acid of high remains unclear in many bacteria. Lactobacillus rhamnosus-10-10-60 SCT previously obtained from L. rhamnosus ATCC 11443 by mutagenesis and showed increased production of L-lactic acid. In this study, genome-10-10-60 SCT strain and ATCC 11443 were sequenced.

The second genome is a circular chromosome, 2.99 Mb long with a GC content of about 46.8%. Eight split genes identified in strain-10-10-60 SCT, including two LytR family of transcriptional regulators, two Rex redox-sensing transcriptional repressor, and four ABC transporters. In total, 60 genes were significantly up-regulated (log2fold-change ≥ 2) and 39 significantly down-regulated genes (log2fold-change ≤ – 2) were identified by transcriptome comparison between SCT-10-10-60 strain and ATCC 11443. KEGG pathway enrichment analysis revealed that “pyruvate metabolism” significantly different (P <0.05) between the two strains.

Anti-CYB5A/Cytochrome b5 Antibody
MBS2401118-005mg 0.05mg
EUR 595

Anti-CYB5A/Cytochrome b5 Antibody
MBS2401118-5x005mg 5x0.05mg
EUR 2500

Anti- Cytochrome P450 1A1 Antibody
GWB-4B4AE1 0.1 ml Ask for price

Anti- Cytochrome P450 2C11 Antibody
GWB-2D2E2C 0.1 ml Ask for price

Anti- Cytochrome b5 Rabbit Antibody
GWB-E6C3AB 0.1 ml Ask for price

Cytochrome c Antibody
ABF7004 100 ug
EUR 525.6

Cytochrome c Antibody
ABF0146 100 ug
EUR 525.6

Cytochrome c Antibody
8C0170 50ug
EUR 368
Description: Cytochrome c Antibody

Cytochrome C Antibody
48927 100ul
EUR 499

Cytochrome C Antibody
48927-100ul 100ul
EUR 399.6

Cytochrome C Antibody
48927-50ul 50ul
EUR 286.8

Cytochrome C Antibody
48976 100ul
EUR 499

Cytochrome C Antibody
48976-100ul 100ul
EUR 399.6

Cytochrome C Antibody
48976-50ul 50ul
EUR 286.8

Cytochrome c Antibody
33354 100ul
EUR 319

Cytochrome c Antibody
33354-100ul 100ul
EUR 302.4

Cytochrome c Antibody
33354-50ul 50ul
EUR 224.4

Cytochrome c antibody
70R-11564 100 ug
EUR 406
Description: Rabbit polyclonal Cytochrome c antibody

Cytochrome c antibody
70R-11766 100 ug
EUR 343
Description: Rabbit polyclonal Cytochrome c antibody

Cytochrome c antibody
70R-11767 100 ug
EUR 343
Description: Rabbit polyclonal Cytochrome c antibody

Cytochrome c antibody
70R-13781 100 ug
EUR 519
Description: Affinity purified Rabbit polyclonal Cytochrome c antibody

Cytochrome C antibody
70R-31158 100 ug
EUR 294
Description: Rabbit polyclonal Cytochrome C antibody

Cytochrome C antibody
70R-51008 100 ul
EUR 242
Description: Purified Polyclonal Cytochrome C antibody

Cytochrome c Antibody
3352-100 each
EUR 379.2

Cytochrome c Antibody
3352-30T each
EUR 175.2

Cytochrome c Antibody
3353-100 each
EUR 379.2

Cytochrome c Antibody
3353-30T each
EUR 175.2

Cytochrome c Antibody
3025-100 each
EUR 438

Cytochrome c Antibody
3025-30T each
EUR 175.2

Cytochrome C antibody
10R-8465 100 ul
EUR 471.6
Description: Mouse monoclonal Cytochrome C antibody

Cytochrome C Antibody
21680 100ul
EUR 319

Cytochrome C Antibody
21680-100ul 100ul
EUR 302.4

Cytochrome c antibody
20R-1430 100 ug
EUR 807.6
Description: Rabbit polyclonal Cytochrome c antibody

Cytochrome c antibody
20R-1518 100 ug
EUR 807.6
Description: Rabbit polyclonal Cytochrome c antibody

Cytochrome c antibody
20R-1519 100 ug
EUR 807.6
Description: Rabbit polyclonal Cytochrome c antibody

Cytochrome C Antibody
21680-50ul 50ul
EUR 224.4

Cytochrome B5 antibody
20R-CG009 100 ul Ask for price
Description: Goat polyclonal Cytochrome B5 antibody

Cytochrome C antibody
10R-6609 100 ug
EUR 398
Description: Mouse monoclonal Cytochrome C antibody

cytochrome b Antibody
39342-100ul 100ul
EUR 468

Cytochrome c Antibody
CSB-PA006328XA01AAF-02mg 0.2mg Ask for price
Description: Recombinant Abutilon theophrasti (Velvet-leaf) (Sida abutilon) Cytochrome c protein

Cytochrome c Antibody
CSB-PA006328XA01AAF-10mg 10mg Ask for price
Description: Recombinant Abutilon theophrasti (Velvet-leaf) (Sida abutilon) Cytochrome c protein

Cytochrome c Antibody
CSB-PA006328XA01FIG-02mg 0.2mg Ask for price
Description: Recombinant Solanum tuberosum (Potato) Cytochrome c protein

Cytochrome c Antibody
CSB-PA006328XA01FIG-10mg 10mg Ask for price
Description: Recombinant Solanum tuberosum (Potato) Cytochrome c protein

Cytochrome c Antibody
CSB-PA006328XA01GEP-02mg 0.2mg Ask for price
Description: Recombinant Gossypium barbadense (Sea-island cotton) (Egyptian cotton) Cytochrome c protein

Cytochrome c Antibody
CSB-PA006328XA01GEP-10mg 10mg Ask for price
Description: Recombinant Gossypium barbadense (Sea-island cotton) (Egyptian cotton) Cytochrome c protein

Cytochrome c Antibody
CSB-PA006328XA01SCK-02mg 0.2mg Ask for price
Description: Recombinant Sarcophaga peregrina (Flesh fly) (Boettcherisca peregrina) Cytochrome c protein

Cytochrome c Antibody
CSB-PA006328XA01SCK-10mg 10mg Ask for price
Description: Recombinant Sarcophaga peregrina (Flesh fly) (Boettcherisca peregrina) Cytochrome c protein

Cytochrome c Antibody
CSB-PA006328XA01ZAX-02mg 0.2mg Ask for price
Description: Recombinant Zea mays (Maize) Cytochrome c protein

Cytochrome c Antibody
CSB-PA006328XA01ZAX-10mg 10mg Ask for price
Description: Recombinant Zea mays (Maize) Cytochrome c protein

Cytochrome b5 Antibody
CSB-PA862097XA01RDF-02mg 0.2mg Ask for price
Description: Recombinant Rhizopus stolonifer (Rhizopus nigricans) Cytochrome b5 protein

Cytochrome b5 Antibody
CSB-PA862097XA01RDF-10mg 10mg Ask for price
Description: Recombinant Rhizopus stolonifer (Rhizopus nigricans) Cytochrome b5 protein

Cytochrome c1 Antibody
C20760-100ul 100μl
EUR 217
Description: Cytochrome c1 Rabbit Polyclonal Antibody

Cytochrome c1 Antibody
C20760-50ul 50μl
EUR 143.5
Description: Cytochrome c1 Rabbit Polyclonal Antibody

Cytochrome b Antibody
C30954-100ul 100μl
EUR 217
Description: Cytochrome b Rabbit Polyclonal Antibody

Cytochrome b Antibody
C30954-50ul 50μl
EUR 143.5
Description: Cytochrome b Rabbit Polyclonal Antibody

Cytochrome b5 Antibody
C13038-100ul 100μl
EUR 217
Description: Cytochrome b5 Rabbit Polyclonal Antibody

Cytochrome b5 Antibody
C13038-50ul 50μl
EUR 143.5
Description: Cytochrome b5 Rabbit Polyclonal Antibody

Cytochrome C Antibody
DF6028 200ul
EUR 420

Cytochrome C Antibody
DF6028-100ul 100ul
EUR 168
Description: WB,IHC,IF/ICC,ELISA(peptide)

Cytochrome C Antibody
DF6028-200ul 200ul
EUR 210
Description: WB,IHC,IF/ICC,ELISA(peptide)

Cytochrome b5 Antibody
abx232186-100g 100 µg
EUR 350

Cytochrome b5 Antibody
abx232186-100ug 100 ug
EUR 577.2

Cytochrome C Antibody
AF0146 200ul
EUR 420

Cytochrome C Antibody
AF0146-100ul 100ul
EUR 168
Description: WB,IHC,IF/ICC,ELISA(peptide)

Cytochrome C Antibody
AF0146-200ul 200ul
EUR 210
Description: WB,IHC,IF/ICC,ELISA(peptide)

Cytochrome C Antibody
E021680 100μg/100μl
EUR 255
Description: Available in various conjugation types.

Cytochrome c Antibody
E033354 100μg/100μl
EUR 255
Description: Available in various conjugation types.

Cytochrome C antibody
BF0714 200ul
EUR 540

Cytochrome C antibody
BF0714-100ul 100ul
EUR 210
Description: WB,IHC

Cytochrome C antibody
BF0714-200ul 200ul
EUR 270
Description: WB,IHC

Cytochrome C antibody
BF0714-50ul 50ul
EUR 150
Description: WB,IHC

Cytochrome C Antibody
AF7004 200ul
EUR 540

Cytochrome C Antibody
AF7004-100ul 100ul
EUR 210
Description: WB,IHC,IF/ICC,ELISA(peptide)

Cytochrome C Antibody
AF7004-200ul 200ul
EUR 270
Description: WB,IHC,IF/ICC,ELISA(peptide)

Cytochrome C Antibody
AF7004-50ul 50ul
EUR 150
Description: WB,IHC,IF/ICC,ELISA(peptide)

Cytochrome b5 Antibody
CSB-PA897598XA01FTD-02mg 0.2mg Ask for price
Description: Recombinant Mortierella alpina (Oleaginous fungus) (Mortierella renispora) Cytochrome b5 protein

Cytochrome b5 Antibody
CSB-PA897598XA01FTD-10mg 10mg Ask for price
Description: Recombinant Mortierella alpina (Oleaginous fungus) (Mortierella renispora) Cytochrome b5 protein

Cytochrome C Antibody
GWB-56035A 0.1 mg Ask for price

Cytochrome C, Antibody
GWB-5AC847 1 ml Ask for price

Cytochrome c Antibody
E11-0170C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

cytochrome b Antibody
E301343 100ug/200ul
EUR 275
Description: Available in various conjugation types.

Cytochrome c1 Antibody
E38PA4382 100ul
EUR 225
Description: Available in various conjugation types.

Cytochrome c Antibody
E38PA6018 100ul
EUR 225
Description: Available in various conjugation types.

Cytochrome c Antibody
E38PA9145 100ul
EUR 225
Description: Available in various conjugation types.

Cytochrome c Antibody
E38PA2689 100ul
EUR 225
Description: Available in various conjugation types.

Cytochrome c Antibody
E18-0146-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Cytochrome c Antibody
E18-0146-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Cytochrome c Antibody
E18-7004-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

Cytochrome c Antibody
E18-7004-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Cytochrome C, Antibody
GWB-83C84A 7 ml Ask for price

Cytochrome C Antibody
GWB-F62EAD 0.1 mg Ask for price

Cytochrome C Antibody
GWB-CE42EA 0.05 mg Ask for price

Cytochrome C Antibody
MBS5311127-01mg 0.1mg
EUR 545

Cytochrome C Antibody
MBS5311127-5x01mg 5x0.1mg
EUR 2400

Cytochrome C Antibody
MBS5311269-01mg 0.1mg
EUR 470

Cytochrome C Antibody
MBS5311269-5x01mg 5x0.1mg
EUR 2080

Cytochrome C Antibody
MBS5311270-01mg 0.1mg
EUR 470

Cytochrome C Antibody
MBS5311270-5x01mg 5x0.1mg
EUR 2080

Cytochrome C Antibody
MBS5312596-01mg 0.1mg
EUR 975

Cytochrome C Antibody
MBS5312596-5x01mg 5x0.1mg
EUR 4245

Cytochrome C Antibody
MBS5315626-01mL 0.1mL
EUR 470

Cytochrome C Antibody
MBS5315626-5x01mL 5x0.1mL
EUR 1955

Cytochrome B5 antibody
MBS534859-INQUIRE INQUIRE Ask for price

Cytochrome c Antibody
MBS853572-01mg 0.1mg
EUR 305

Cytochrome c Antibody
MBS853572-01mLAF405L 0.1mL(AF405L)
EUR 465

Cytochrome c Antibody
MBS853572-01mLAF405S 0.1mL(AF405S)
EUR 465

Cytochrome c Antibody
MBS853572-01mLAF610 0.1mL(AF610)
EUR 465

Cytochrome c Antibody
MBS853572-01mLAF635 0.1mL(AF635)
EUR 465

Cytochrome c Antibody
MBS7138755-INQUIRE INQUIRE Ask for price

Cytochrome c Antibody
MBS7138757-INQUIRE INQUIRE Ask for price

Cytochrome c Antibody
MBS7138758-INQUIRE INQUIRE Ask for price

Cytochrome c Antibody
MBS7138760-INQUIRE INQUIRE Ask for price

Cytochrome c Antibody
MBS7138762-INQUIRE INQUIRE Ask for price

Cytochrome c Antibody
MBS8510641-005mg 0.05mg
EUR 235

Cytochrome c Antibody
MBS8510641-01mg 0.1mg
EUR 305

Cytochrome c Antibody
MBS8510641-01mLAF405M 0.1mL(AF405M)
EUR 465

Cytochrome c Antibody
MBS8510641-01mLAF546 0.1mL(AF546)
EUR 465

Cytochrome c Antibody
MBS8510641-01mLAF750 0.1mL(AF750)
EUR 465

Cytochrome c Antibody
MBS8512503-005mg 0.05mg
EUR 235

Cytochrome c Antibody
MBS8512503-01mg 0.1mg
EUR 305

Cytochrome c Antibody
MBS8512503-01mLAF405M 0.1mL(AF405M)
EUR 465

Cytochrome c Antibody
MBS8512503-01mLAF546 0.1mL(AF546)
EUR 465

Cytochrome c Antibody
MBS8512503-01mLAF750 0.1mL(AF750)
EUR 465

cytochrome b Antibody
MBS9403598-01mL 0.1mL
EUR 420

cytochrome b Antibody
MBS9403598-5x01mL 5x0.1mL
EUR 1740

Cytochrome c Antibody
MBS9201868-01mL 0.1mL
EUR 425

Cytochrome c Antibody
MBS9201868-5x01mL 5x0.1mL
EUR 1887

Cytochrome c Antibody
MBS8581490-01mL 0.1mL
EUR 305

Cytochrome c Antibody
MBS8581490-01mLAF405L 0.1mL(AF405L)
EUR 465

Cytochrome c Antibody
MBS8581490-01mLAF405S 0.1mL(AF405S)
EUR 465

Cytochrome c Antibody
MBS8581490-01mLAF610 0.1mL(AF610)
EUR 465

Cytochrome c Antibody
MBS8581490-01mLAF635 0.1mL(AF635)
EUR 465

Cytochrome c Antibody
MBS8584316-01mL 0.1mL
EUR 305

Cytochrome c Antibody
MBS8584316-01mLAF405L 0.1mL(AF405L)
EUR 465

Cytochrome c Antibody
MBS8584316-01mLAF405S 0.1mL(AF405S)
EUR 465

Cytochrome c Antibody
MBS8584316-01mLAF610 0.1mL(AF610)
EUR 465

Cytochrome c Antibody
MBS8584316-01mLAF635 0.1mL(AF635)
EUR 465

cytochrome b Antibody
MBS855703-01mg 0.1mg
EUR 345

cytochrome b Antibody
MBS855703-01mLAF405L 0.1mL(AF405L)
EUR 565

cytochrome b Antibody
MBS855703-01mLAF405S 0.1mL(AF405S)
EUR 565

cytochrome b Antibody
MBS855703-01mLAF610 0.1mL(AF610)
EUR 565

cytochrome b Antibody
MBS855703-01mLAF635 0.1mL(AF635)
EUR 565

Cytochrome b5 Antibody
MBS9006998-INQUIRE INQUIRE Ask for price

Cytochrome b5 Antibody
MBS9015664-INQUIRE INQUIRE Ask for price

Cytochrome c Antibody
MBS8583707-01mL 0.1mL
EUR 305

Cytochrome c Antibody
MBS8583707-01mLAF405L 0.1mL(AF405L)
EUR 465

Cytochrome c Antibody
MBS8583707-01mLAF405S 0.1mL(AF405S)
EUR 465

Cytochrome c Antibody
MBS8583707-01mLAF610 0.1mL(AF610)
EUR 465

Cytochrome c Antibody
MBS8583707-01mLAF635 0.1mL(AF635)
EUR 465

Cytochrome c1 Antibody
MBS8582472-01mL 0.1mL
EUR 305

Cytochrome c1 Antibody
MBS8582472-01mLAF405L 0.1mL(AF405L)
EUR 465

Cytochrome c1 Antibody
MBS8582472-01mLAF405S 0.1mL(AF405S)
EUR 465

Cytochrome c1 Antibody
MBS8582472-01mLAF610 0.1mL(AF610)
EUR 465

Cytochrome c1 Antibody
MBS8582472-01mLAF635 0.1mL(AF635)
EUR 465

Cytochrome c Antibody
MBS9504574-005mL 0.05mL
EUR 285

Cytochrome c Antibody
MBS9504574-01mL 0.1mL
EUR 385

Cytochrome c Antibody
MBS9504574-5x01mL 5x0.1mL
EUR 1590

Cytochrome c Antibody
MBS9600084-01mL 0.1mL
EUR 260

Cytochrome c Antibody
MBS9600084-02mL 0.2mL
EUR 305

Cytochrome c Antibody
MBS9600084-5x02mL 5x0.2mL
EUR 1220

Cytochrome c Antibody
MBS9610366-005mL 0.05mL
EUR 245

Cytochrome c Antibody
MBS9610366-01mL 0.1mL
EUR 305

Cytochrome c Antibody
MBS9610366-02mL 0.2mL
EUR 365

Cytochrome c Antibody
MBS9610366-5x02mL 5x0.2mL
EUR 1495

Gen split and differentially expressed genes involved in “pyruvate metabolism” pathway may be responsible for increasing the production of L-lactic acid by SCT-10-10-60. Genome and transcriptome sequencing information and comparison of SCT-10-10-60 with ATCC 11443 gives an insight into the L-lactic acid anabolism and references to increase the production of L-lactic acid using genetic engineering.