Microbial Genomics of the Global Ocean System: Report on an American Academy of Microbiology (Academy), The American Geophysical Union (AGU), and The Gulf of Mexico Research Initiative (GoMRI) Colloquium held on 9 and 10 April 2019
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Microbial Genomics of the Global Ocean System: Report on an American Academy of Microbiology (Academy), The American Geophysical Union (AGU), and The Gulf of Mexico Research Initiative (GoMRI) Colloquium held on 9 and 10 April 2019
2020 marks the 10th anniversary of the Deepwater Horizon (DWH) disaster. From April to July 2010, an estimated total of 4.9 million barrels of oil and 250,000 metric tons of natural gas that is discharged into the Gulf of Mexico. Not only eleven lives lost, but the tragedy was also left a lasting impact on the Gulf marine and coastal ecosystems and the people who depend on these habitats for their livelihoods. After the oil spill, the Gulf of Mexico microbial communities play an important role in the cleanup, bioremediation of hydrocarbons core service contributions.
Despite its importance, marine microbiology hydrocarbon is a young field. Before the spill relatively little is known about marine hydrocarbon degraders. Starting in 2010, the development and application of genomics and bioinformatics tools allow researchers – for the first time – to identify and examine individual microorganisms in a complex society them in detail unprecedented. Today, technical advances and new discoveries reveal the natural capacity of the microbes in the Gulf of Mexico to catalyze the bioremediation of petroleum hydrocarbons.
This knowledge is essential to guide mitigation and recovery strategies that build on the ability of the natural bioremediation microbes without further disrupt sensitive ecosystems. This report is based on the consideration of experts who participated in a colloquium together with the American Academy of Microbiology, ASM honor the leadership group, the American Geophysical Union (AGU), and the Gulf of Mexico Research Initiative (GoMRI) in April 2019. The report highlights new tools research, methodology, data sources, collaboration, and models that will advance basic and applied research to deliver data-driven solutions to environmental challenges.
Microbial Genomics of the Global Ocean System: Report on an American Academy of Microbiology (Academy), The American Geophysical Union (AGU), and The Gulf of Mexico Research Initiative (GoMRI) Colloquium held on 9 and 10 April 2019
Estimating allele-Specific Expression of SNVs From the 10 x Genomics Single-cell RNA-Sequencing data.
With recent advances in single-cell RNA-sequencing (scRNA-seq) technology, estimation of allele expression of a single cell becomes more reliable. allele expression is both quantitative and dynamic and is an important component of interactome genome. Here, we systematically estimate the expression of alleles from heterozygous single nucleotide variants (SNV) locus scRNA-seq using the data generated on the platform 10 × Genomics Chromium.
We analyzed 26 640 human adipose-derived mesenchymal stem cells (from three healthy donors), sorted by an average of 150K sequencing reads per cell (more than 4 billion scRNA-seq reads in total). SNV high quality calls assessed in our study contains about 15% exonic and> 50% of intronic loci. To analyze the expression of alleles, we estimate allelic variants declare fraction (VAFRNA) of SNV-conscious bias and variance analysis and distribution (mono- and bi-allele) in a different sequence reads minimum threshold.
Description: A polyclonal antibody against OXR1. Recognizes OXR1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200
Description: A polyclonal antibody against OXR1. Recognizes OXR1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100
Description: A polyclonal antibody against OXR1. Recognizes OXR1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100
Description: A polyclonal antibody against OXR1. Recognizes OXR1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/40000
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human OXR1 . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody for detection of Oxr1 from Human, Mouse, Rat. This Oxr1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Oxr1 at AA range: 450-530
Description: A polyclonal antibody for detection of Oxr1 from Human, Mouse, Rat. This Oxr1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Oxr1 at AA range: 450-530
Description: A polyclonal antibody for detection of Oxr1 from Human, Mouse, Rat. This Oxr1 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Oxr1 at AA range: 450-530
Description: A polyclonal antibody against OXR1. Recognizes OXR1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against OXR1. Recognizes OXR1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against OXR1. Recognizes OXR1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: Description of target: May be involved in protection from oxidative damage. ;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Competitive ELISA;Sensitivity: 0.317 ng/mL
Description: Description of target: May be involved in protection from oxidative damage.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Competitive ELISA;Sensitivity: 0.327 ng/mL
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-OX1R and OX2R . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody for detection of OX2R from Human. This OX2R antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human OX2R at AA range: 210-290
Description: A polyclonal antibody for detection of OX2R from Human. This OX2R antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human OX2R at AA range: 210-290
Description: A polyclonal antibody for detection of OX2R from Human. This OX2R antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human OX2R at AA range: 210-290
Description: A polyclonal antibody for detection of OX2R from Human, Mouse, Rat. This OX2R antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human OX2R protein at amino acid sequence of 280-360
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Our analysis shows that when judging the closed position by at least three unique sequencing reads, more than 50% of heterozygous SNVs bi-allelic expression, while at the threshold 10 reads, almost 90% of bi-allelic SNVs. In addition, we demonstrate the feasibility analysis estimates scVAFRNA of scRNA-Seq datasets present and showed that the 3′-generation protocol library based on 10 data × Genomics scRNA-seq can be informative in SNV-based studies, including analysis of the kinetics of transcription.
