Clonal Dissemination of Pseudomonas aeruginosa Sequence Type 235 Isolates Carrying blaIMP-6 and Emergence of blaGES-24 and blaIMP-10 on Novel Genomic Islands PAGI-15 and -16 in South Korea.
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Clonal Dissemination of Pseudomonas aeruginosa Sequence Type 235 Isolates Carrying blaIMP-6 and Emergence of blaGES-24 and blaIMP-10 on Novel Genomic Islands PAGI-15 and -16 in South Korea.
A total of 431 clinical isolates of Pseudomonas aeruginosa were collected from 29 public hospitals in South Korea in 2015. Antimicrobial susceptibility was tested by disk diffusion method and MIC of carbapenems are determined by dilution method in order. Carbapenemase gene amplified by PCR and sequenced, and the structure of class 1 integrons about carbapenemase gene cassette were analyzed by PCR mapping. Multilokus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) is made to the type of virus typing. Whole-genome sequencing was conducted to analyze the genome of P. aeruginosa island (Pagis) carries blaIMP-6, blaIMP-10, and blaGES-24 gene. Carbapenem-nonsusceptible level and carbapenemase-producing isolates of P. aeruginosa was 34.3% (148/431) and 9.5% (41/431), respectively.
IMP-6 is the most common type of carbapenemase, followed by VIM-2, IMP-10, and GES-24. All carbapenemase gene located on class 1 integrons of 6 different types on chromosomes. All isolates store carbapenemase gene exhibited genetic relatedness by PFGE (similarity >> 80%); In addition, all isolates were identified as order type 235 (ST235), with the exception of two ST244 isolates by MLST. The blaIMP-6, blaIMP-10, and blaGES-24 genes were found to be at two new Pagis, designated MORNING MORNING 15th and 16th. Our data support the clonal dissemination of IMP-6-producing P. aeruginosa strain ST235, and the emergence of the IMP-10 and GES-24 shows the diversification carbapenemases in P. aeruginosa in Korea.
Lactobacillus pentosus MP-10, which is isolated from the salty water from the natural fermentation of green table olives Aloreña, exhibited high potency probiotic. The genome sequence of L. pentosus MP-10 is now considered the largest genome among the lactobacilli, highlighting the flexibility and adaptability of microorganisms ecology. Here, we analyzed the complete genome sequences obtained for the presence of antibiotic resistance and virulence determinants to understand the mechanism of their defense and security explore alleged in food.
The genome sequence reveals evidence described a variety of mobile genetic elements, such as prophages, transposases and transposons are involved in their adaptation to salt water-related niches. In silico analysis of L. pentosus MP-10 genome sequence to identify CRISPR (clustered regularly interspaced short palindromic repeats) / cas (CRISPR associated protein gene) as the immune system against foreign genetic elements
Clonal Dissemination of Pseudomonas aeruginosa Sequence Type 235 Isolates Carrying blaIMP-6 and Emergence of blaGES-24 and blaIMP-10 on Novel Genomic Islands PAGI-15 and -16 in South Korea.
Role of N-α-acetyltransferase 10 Protein in DNA Methylation and Genomic Inclusion.
genomic imprinting is the phenomenon of gene expression is mainly controlled by the allele allele-specific DNA methylation in the printing control region (ICR), but the underlying mechanisms are still largely unclear. N-α-acetyltransferase protein 10 (Naa10p) catalyzes the N-α-acetylated nascent proteins, and mutations in human Naa10p associated with severe developmental delay.
Here we report that Naa10-null mice displaying partial embryonic lethal, growth retardation, cerebral palsy and the lethal effects of the mother, a common phenotype observed in genomic imprinting damaged. Genome analysis further reveals global DNA hypomethylation and dysregulation of imprinted genes enriched in Naa10p-KO embryos and embryonic stem cells.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.
Mechanically, Naa10p facilitate binding to DNA methyltransferase 1 (DNMT1) to the DNA substrates, including imprinted allele ICRS during the phase S. In addition, Ogden deadly human Naa10p related syndrome mutations disrupt the binding to the ICR of H19 and DNMT1 recruitment. Our study thus links Naa10p Ogden syndrome related mutations for damaged DNA methylation and genomic imprinting.
