Complete genome sequences of pooled genomic DNA from 10 marine bacteria using PacBio long-read sequencing.

High-quality, complete genome is important to understand the functioning of marine bacteria. PacBio sequencing technology provides a powerful way to get a high quality finished genome. However, the production of individual library is still expensive, limiting the utility of PacBio system for high-throughput genomics. Here we investigate how to generate high-quality genome of the marine bacterium pooled genomic DNA from 10 bacterial genomes.Pooled sea into a single library production targets and sequencing with eight cells in the SMRT sequencing platform PacBio RS II. In total, 7.35 Gbp read the old data is generated, which is equivalent to 168 × coverage median forecast for input genome.

Genome assembly showed that eight genome with an average nucleotide identity (ANI) lower than 91.4% can be assembled with high quality and resolution using standard assembly algorithm (eg hgap or Canu). A staged reading based reference were developed and included steps to assemble a complete genome of the marine bacterium remaining two who had ANI> 97% and the beginning of a very fragmented.Ten genome assemblies complete high-quality marine bacteria produced. The findings and developments are made here, including staged reading based approaches for genome assembly references are very similar, can be used in the future to devise a strategy to sequence the genomes collected using the long-read sequencing.


Massively parallel DNA sequencing an opportunity to bridge some of the temporal and spatial dimensions in biodiversity research, thanks to the efficiency to recover millions of nucleotide polymorphisms. Here, we identify the current status, discusses the main challenges, and look into the future perspectives on biodiversity genomics focuses on the insect, which is arguably the most diverse group and ecologically important among all animals.

We suggest 10 simple rules that provide a quick step-by-step guidance and best practices for anyone who is interested in the study of biodiversity through genomic studies insects. To this end, we review the relevant literature on biodiversity and evolutionary research in the field of entomology.

 Complete genome sequences of pooled <em>genomic</em> DNA from <em>10</em> marine bacteria using PacBio long-read sequencing.
Complete genome sequences of pooled genomic DNA from 10 marine bacteria using PacBio long-read sequencing.

(Cyto) genome and epigenetic characterization of BICR 10 three established cell lines and primary human head and neck squamous cell carcinoma new culture.

Head and neck squamous cell carcinoma cell lines are useful preclinical model for understanding the molecular processes that underlie the development of tumors, and to build targeted therapies.We do (cyto) a comprehensive characterization of genomic and epigenetic newly established three primary human head and neck squamous cell carcinoma cultures and well-established, but undercharacterized cell line: BICR 10.Karyotyping, multiplex fluorescence in situ hybridization, various comparative genomics and methylation hybridization particular multiplex ligation dependent probe amplification applied.The three main culture turned out to be a near-triploid and BICR 10 near-diploid. Appeals and molecular cytogenetic analysis revealed non-random numerical and structural aberrations.

RPL40 Antibody

34362-50ul 50ul
EUR 224.4

RPL40 Antibody

E034362 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL40 Antibody

E11-14185C 100μg
EUR 225
Description: Available in various conjugation types.

RPL40 antibody

70R-33962 100 ug
EUR 294
Description: Rabbit polyclonal RPL40 antibody

RPL40 Antibody

abx014130-100l 100 µl
EUR 43.75

RPL40 Conjugated Antibody

C34362 100ul
EUR 476.4

Rabbit polyclonal anti-RPL40 / UBA52 antibody

TA313017 100 µl Ask for price

anti- RPL10 antibody

FNab07408 100µg
EUR 658.5
Description: Antibody raised against RPL10

anti- RPL11 antibody

FNab07410 100µg
EUR 658.5
Description: Antibody raised against RPL11

anti- RPL12 antibody

FNab07411 100µg
EUR 658.5
Description: Antibody raised against RPL12

anti- RPL13 antibody

FNab07412 100µg
EUR 658.5
Description: Antibody raised against RPL13

anti- RPL14 antibody

FNab07414 100µg
EUR 658.5
Description: Antibody raised against RPL14

anti- RPL15 antibody

FNab07415 100µg
EUR 658.5
Description: Antibody raised against RPL15

anti- RPL17 antibody

FNab07416 100µg
EUR 658.5
Description: Antibody raised against RPL17

anti- RPL19 antibody

FNab07419 100µg
EUR 658.5
Description: Antibody raised against RPL19

anti- RPL21 antibody

FNab07420 100µg
EUR 658.5
Description: Antibody raised against RPL21

anti- RPL22 antibody

FNab07421 100µg
EUR 702
Description: Antibody raised against RPL22

anti- RPL23 antibody

FNab07422 100µg
EUR 658.5
Description: Antibody raised against RPL23

anti- RPL24 antibody

FNab07424 100µg
EUR 658.5
Description: Antibody raised against RPL24

anti- RPL27 antibody

FNab07426 100µg
EUR 658.5
Description: Antibody raised against RPL27

anti- RPL28 antibody

FNab07428 100µg
EUR 658.5
Description: Antibody raised against RPL28

anti- RPL30 antibody

FNab07431 100µg
EUR 658.5
Description: Antibody raised against RPL30

anti- RPL31 antibody

FNab07432 100µg
EUR 658.5
Description: Antibody raised against RPL31

anti- RPL32 antibody

FNab07433 100µg
EUR 658.5
Description: Antibody raised against RPL32

anti- RPL35 antibody

FNab07434 100µg
EUR 658.5
Description: Antibody raised against RPL35

anti- RPL38 antibody

FNab07437 100µg
EUR 658.5
Description: Antibody raised against RPL38

anti- RPL39 antibody

FNab07438 100µg
EUR 658.5
Description: Antibody raised against RPL39

anti- RPL29 antibody

FNab09831 100µg
EUR 658.5
Description: Antibody raised against RPL29

anti- RPL29 antibody

LSMab09831 100 ug
EUR 463.2

anti- RPL10A antibody

FNab07409 100µg
EUR 658.5
Description: Antibody raised against RPL10A

anti- RPL13A antibody

FNab07413 100µg
EUR 658.5
Description: Antibody raised against RPL13A

anti- RPL18A antibody

FNab07417 100µg
EUR 658.5
Description: Antibody raised against RPL18A

anti- RPL18A antibody

FNab07418 100µg
EUR 658.5
Description: Antibody raised against RPL18A

anti- RPL23A antibody

FNab07423 100µg
EUR 658.5
Description: Antibody raised against RPL23A

anti- RPL27A antibody

FNab07427 100µg
EUR 658.5
Description: Antibody raised against RPL27A

anti- RPL37A antibody

FNab07436 100µg
EUR 658.5
Description: Antibody raised against RPL37A

anti- RPL39L antibody

FNab07439 100µg
EUR 702
Description: Antibody raised against RPL39L

anti- RPL26L1 antibody

FNab07425 100µg
EUR 658.5
Description: Antibody raised against RPL26L1

anti- RPL36AL antibody

FNab07435 100µg
EUR 702
Description: Antibody raised against RPL36AL

anti- RPL3 antibody

FNab07429 100µg
EUR 658.5
Description: Antibody raised against RPL3

anti- RPL3 antibody

FNab07430 100µg
EUR 702
Description: Antibody raised against RPL3

anti- RPL5 antibody

FNab07440 100µg
EUR 658.5
Description: Antibody raised against RPL5

anti- RPL6 antibody

FNab07441 100µg
EUR 658.5
Description: Antibody raised against RPL6

anti- RPL7 antibody

FNab07442 100µg
EUR 658.5
Description: Antibody raised against RPL7

anti- RPL8 antibody

FNab07445 100µg
EUR 658.5
Description: Antibody raised against RPL8

anti- RPL9 antibody

FNab07446 100µg
EUR 658.5
Description: Antibody raised against RPL9

anti- RPL7A antibody

FNab07443 100µg
EUR 658.5
Description: Antibody raised against RPL7A

anti- RPL7L1 antibody

FNab07444 100µg
EUR 658.5
Description: Antibody raised against RPL7L1

Anti-Ribosomal Protein L19 / RPL19 antibody

STJ70939 100 µg
EUR 430.8

Goat Anti-Ribosomal Protein L19 / RPL19 Antibody

TA311149 100 µg Ask for price

Goat anti-Ribosomal Protein L19 / RPL19 Antibody

dAP-0895 50ug
EUR 264.6

anti- RPLP0 antibody

FNab07447 100µg
EUR 658.5
Description: Antibody raised against RPLP0

anti- RPLP0 antibody

FNab07448 100µg
EUR 702
Description: Antibody raised against RPLP0

anti- RPLP1 antibody

FNab07449 100µg
EUR 702
Description: Antibody raised against RPLP1

Polyclonal Goat Anti-Ribosomal Protein L19 / RPL19 Antibody

APG00278G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-Ribosomal Protein L19 / RPL19 . This antibody is tested and proven to work in the following applications:

RPL31 Antibody

ABD13238 100 ug
EUR 525.6

RPL35 Antibody

ABD13239 100 ug
EUR 525.6

RPL12 Antibody

8C14148 50ug
EUR 368
Description: RPL12 Antibody

RPL15 Antibody

8C14152 50ug
EUR 368
Description: RPL15 Antibody

RPL17 Antibody

8C14153 50ug
EUR 368
Description: RPL17 Antibody

RPL18 Antibody

8C14154 50ug
EUR 368
Description: RPL18 Antibody

RPL22 Antibody

8C14158 50ug
EUR 368
Description: RPL22 Antibody

RPL23 Antibody

8C14159 50ug
EUR 368
Description: RPL23 Antibody

RPL28 Antibody

8C14166 50ug
EUR 368
Description: RPL28 Antibody

RPL30 Antibody

8C14169 50ug
EUR 368
Description: RPL30 Antibody

RPL31 Antibody

8C14170 50ug
EUR 368
Description: RPL31 Antibody

RPL34 Antibody

8C14172 50ug
EUR 368
Description: RPL34 Antibody

RPL35 Antibody

8C14173 50ug
EUR 368
Description: RPL35 Antibody

RPL36 Antibody

8C14175 50ug
EUR 368
Description: RPL36 Antibody

RPL37 Antibody

8C14178 50ug
EUR 368
Description: RPL37 Antibody

RPL39 Antibody

8C14181 50ug
EUR 368
Description: RPL39 Antibody

RPL29 Antibody

20-abx124394
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul

RPL41 Antibody

20-abx147791
  • Ask for price
  • Ask for price
  • 100 ug
  • 50 ug

RPL29 Antibody

abx034814-400ul 400 ul
EUR 627.6

RPL29 Antibody

abx034814-80l 80 µl
EUR 343.2

RPL14 antibody

22339 100ul
EUR 479

RPL14 antibody

22339-100ul 100ul
EUR 468

RPL28 Antibody

47712 100ul
EUR 319

RPL28 Antibody

47712-100ul 100ul
EUR 302.4

RPL22 Antibody

47906 100ul
EUR 319

RPL22 Antibody

47906-100ul 100ul
EUR 302.4

RPL37 Antibody

45733 100ul
EUR 319

RPL37 Antibody

45733-100ul 100ul
EUR 302.4

RPL37 Antibody

45733-50ul 50ul
EUR 224.4

RPL38 Antibody

45734-100ul 100ul
EUR 302.4

RPL38 Antibody

45734-50ul 50ul
EUR 224.4

RPL41 Antibody

45736-100ul 100ul
EUR 302.4

RPL41 Antibody

45736-50ul 50ul
EUR 224.4

RPL24 Antibody

45729-100ul 100ul
EUR 302.4

RPL24 Antibody

45729-50ul 50ul
EUR 224.4

RPL27 Antibody

45730-100ul 100ul
EUR 302.4

RPL27 Antibody

45730-50ul 50ul
EUR 224.4

RPL29 Antibody

45731-100ul 100ul
EUR 302.4

RPL29 Antibody

45731-50ul 50ul
EUR 224.4

RPL12 Antibody

34346 100ul
EUR 319

RPL12 Antibody

34346-100ul 100ul
EUR 302.4

RPL12 Antibody

34346-50ul 50ul
EUR 224.4

RPL14 Antibody

34347 100ul
EUR 319

RPL14 Antibody

34347-100ul 100ul
EUR 302.4

RPL14 Antibody

34347-50ul 50ul
EUR 224.4

RPL15 Antibody

34348 100ul
EUR 319

RPL15 Antibody

34348-100ul 100ul
EUR 302.4

RPL15 Antibody

34348-50ul 50ul
EUR 224.4

RPL18 Antibody

34349 100ul
EUR 319

RPL18 Antibody

34349-100ul 100ul
EUR 302.4

RPL18 Antibody

34349-50ul 50ul
EUR 224.4

RPL28 Antibody

34352 100ul
EUR 319

RPL28 Antibody

34352-100ul 100ul
EUR 302.4

RPL28 Antibody

34352-50ul 50ul
EUR 224.4

RPL30 Antibody

34354 100ul
EUR 319

RPL30 Antibody

34354-100ul 100ul
EUR 302.4

RPL30 Antibody

34354-50ul 50ul
EUR 224.4

RPL31 Antibody

34355 100ul
EUR 319

RPL31 Antibody

34355-100ul 100ul
EUR 302.4

RPL31 Antibody

34355-50ul 50ul
EUR 224.4

RPL34 Antibody

34356 100ul
EUR 319

RPL34 Antibody

34356-100ul 100ul
EUR 302.4

RPL34 Antibody

34356-50ul 50ul
EUR 224.4

RPL35 Antibody

34357 100ul
EUR 319

RPL35 Antibody

34357-100ul 100ul
EUR 302.4

RPL35 Antibody

34357-50ul 50ul
EUR 224.4

RPL36 Antibody

34358 100ul
EUR 319

RPL36 Antibody

34358-100ul 100ul
EUR 302.4

RPL36 Antibody

34358-50ul 50ul
EUR 224.4

RPL39 Antibody

34359 100ul
EUR 319

RPL39 Antibody

34359-100ul 100ul
EUR 302.4

RPL39 Antibody

34359-50ul 50ul
EUR 224.4

RPL11 Antibody

42741 100ul
EUR 319

RPL11 Antibody

42741-100ul 100ul
EUR 302.4

RPL11 antibody

38886 100ul
EUR 439

RPL11 antibody

38886-100ul 100ul
EUR 302.4

RPL13 antibody

39130-100ul 100ul
EUR 302.4

RPL14 antibody

39131 100ul
EUR 439

RPL14 antibody

39131-100ul 100ul
EUR 302.4

RPL29 Antibody

20-abx218356
  • Ask for price
  • Ask for price
  • 100 ug
  • 50 ug

RPL14 Antibody

CSB-PA961640- each
EUR 402
Description: A polyclonal antibody against RPL14. Recognizes RPL14 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

RPL14 Antibody

CSB-PA961640-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against RPL14. Recognizes RPL14 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

RPL35 Antibody

CSB-PA980122- each
EUR 402
Description: A polyclonal antibody against RPL35. Recognizes RPL35 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

RPL35 Antibody

CSB-PA980122-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against RPL35. Recognizes RPL35 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

RPL30 Antibody

CSB-PA814533- each
EUR 402
Description: A polyclonal antibody against RPL30. Recognizes RPL30 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

RPL30 Antibody

CSB-PA814533-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against RPL30. Recognizes RPL30 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

RPL12 Antibody

E034346 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL14 Antibody

E034347 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL15 Antibody

E034348 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL18 Antibody

E034349 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL28 Antibody

E034352 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL30 Antibody

E034354 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL31 Antibody

E034355 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL34 Antibody

E034356 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL35 Antibody

E034357 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL36 Antibody

E034358 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL39 Antibody

E034359 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL28 Antibody

E047712 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL22 Antibody

E047906 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL11 Antibody

E042741 100μg/100μl
EUR 255
Description: Available in various conjugation types.

RPL11 Antibody

E11-14147C 100μg
EUR 225
Description: Available in various conjugation types.

RPL12 Antibody

E11-14148C 100μg
EUR 225
Description: Available in various conjugation types.

RPL14 Antibody

E11-14151C 100μg
EUR 225
Description: Available in various conjugation types.

RPL15 Antibody

E11-14152C 100μg
EUR 225
Description: Available in various conjugation types.

RPL17 Antibody

E11-14153C 100μg
EUR 225
Description: Available in various conjugation types.

RPL18 Antibody

E11-14154C 100μg
EUR 225
Description: Available in various conjugation types.

RPL19 Antibody

E11-14156C 100μg
EUR 225
Description: Available in various conjugation types.

The most common rearrangements were identified in BICR 10 cell lines are derivatives of non-reciprocal translocation complex, where breakpoints frequently appears in / near-centromeric centromeric regions. In 3 Primary cell cultures are most commonly observed rearrangements are iso- and derivatives derived from translocated chromosomes. Overall, the advantages of 7p, 8Q and losses at 3p, 8p, 9p, 18q and Xp is present in all four samples studied. Among the genes analyzed, BICR line 10 cells exhibited enhanced gene promoter methylation; However, in all the samples studied PAX5, WT1 and GATA5 were methylated.